Press the Start button. The speed display will gradually climb to your programmed RPM. Unloading Samples
The 5402 does not calculate relative centrifugal force (RCF) automatically. You need the manual’s rotor-specific radius values to manually convert rpm to g. For pelleting RNA or separating subcellular fractions, that formula is non-negotiable.
, it is crucial to read the original operating manual thoroughly to ensure safety and prevent equipment damage.
Turn the timer knob to the desired duration, or set it to "Hold" for manual timing. Running a Cycle Load samples and secure the rotor lid. eppendorf 5402 manual
Pre-cools the rotor chamber quickly before temperature-sensitive runs. Short Spin Button: For quick, momentary spins (pulldowns).
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: Modern centrifugation relies on the strict maintenance of center-of-mass alignment. The paper discusses the physics of rotor imbalance—where even a 0.1g variance at 14,000 RPM translates into significant destructive torque. The RCF Equation Press the Start button
Why this matters: Exceeding 14,000 rpm with a non-rated rotor is explicitly forbidden in the manual.
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Turn the device on and set the desired temperature using the cooling control button. Allow the unit to reach the temperature before starting the run. 3.2 Loading the Rotor Open Lid: Press the lid release button. You need the manual’s rotor-specific radius values to
The Eppendorf 5402 offers a range of innovative features that make it an exceptional centrifuge:
Place the unit on a stable, level, and vibration-free laboratory bench.